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Microglia Phagocytosis

Phagocytosis is a key function of microglia/macrophage for the removal of pathogens, debris and dead or apoptotic cells in homeostasis and following injury or infection. Dysfunctional macrophage phagocytosis has been implicated in many inflammatory conditions including neuroinflammation, respiratory disease, rheumatoid arthritis and more.

We use an array of pHrodo labelled baits (S. aureus, E. coli, zymosan beads) and can label proteins, beta-amyloid (1-42), alpha-synuclein and dead cells, making this assay highly customisable.

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Phagocytosis video:

Phagocytosis of pHrodo labelled S. aureus by iPSC-derived microglia-like cells. Cells were incubated with S. aureus and fluorescence was measured over a 25-hour time period on an IncuCyte S3.

Phagocytosis over time graph:

Phagocytosis of pHrodo labelled S. aureus by iPSC-derived microglia-like cells over a 25-hour time period on an IncuCyte S3.

Confocal image of phagocytosis:

Phagocytosis of pHrodo labelled S. aureus by iPSC-derived microglia after 4h incubation. Blue: DAPI, Red: S. aureus, Green: CellTracker.