Neurobiology Sensory neurons
Sensory neurons detect numerous external stimuli. Censo’s human iPSC-derived sensory neurons express nociception-relevant markers upon maturation, which make them useful for the study of pain. Once mature, these neurons also express frataxin, which when altered, is the cause of Friedreich’s Ataxia.
Human iPSC derived sensory neurons can be used to study an array of conditions and are amenable to a range of assays, such as neurite outgrowth and calcium release in response to compound treatment.
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iPSCs were differentiated to sensory precursors over 12 days. At this point, upregulation of sensory markers such as Brn3a and Isl1-1 and nociceptive genes such as Ntrk1 and Scn10a is observed. After longer maturation, commonly to a total of 46 days, other pain receptors are expressed e.g. Scn9a. Meanwhile, expression of neuroectodermal marker Otx2 substantially decreases.
iPSC-derived sensory neurons at day 46: neuronal marker TUJ-1 (Green), sensory marker BRN3A (Red) and Nuclei (Blue).
iPSC-derived sensory neurons at day 46: neuronal marker TUJ-1 (Green), peripheral marker SOX10 (Red) and Nuclei (Blue).