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Differentiation models Microglia and macrophages

Microglia are a specialized population of macrophages in the central nervous system (CNS) and are capable of organizing a potent inflammatory response. They are responsible of synaptic reorganization, phagocytosis of apoptotic cells in the developing brain, myelin turnover, and control of neuronal excitability. Differentiation of iPSC to microglia/macrophage-like cells is achieved via mesoderm induction and embryonic body formation. This process is easily scaled up and has been performed on over 18 iPSC lines with highly reproducible batch-to-batch results. These cells have undergone extensive characterization including flow cytometry, ICC, transcriptomic analysis, and functional assays.

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Flow diagram of cell differentiation:

Flow diagram of the differentiation process from iPSC-to microglia/macrophage-like cells. Differentiation is performed by mesoderm induction and embryonic body formation, with cells produced for up to 4 months.

Macrophage-like
Microglia-like

Phase contrast images:

Phase contrast images of iPSC derived microglia- and macrophage-like cells following 7 day maturation.

CD33

CX3CR1

P2RY12

TREM2

Immunocytochemistry images:

Immunocytochemistry of iPSC-derived microglia-like cells stained for classic markers.

Flow Cytometry:

Cell surface receptor expression measured by flow cytometry. Each batch of cells generated is quality control tested by measuring expression of cell surface receptors.

Purple: Isotype
Blue: Marker of interest