Differentiation models Microglia and macrophages
Microglia are a specialized population of macrophages in the central nervous system (CNS) and are capable of organizing a potent inflammatory response. They are responsible of synaptic reorganization, phagocytosis of apoptotic cells in the developing brain, myelin turnover, and control of neuronal excitability. Differentiation of iPSC to microglia/macrophage-like cells is achieved via mesoderm induction and embryonic body formation. This process is easily scaled up and has been performed on over 18 iPSC lines with highly reproducible batch-to-batch results. These cells have undergone extensive characterization including flow cytometry, ICC, transcriptomic analysis, and functional assays.
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Flow diagram of cell differentiation:
Flow diagram of the differentiation process from iPSC-to microglia/macrophage-like cells. Differentiation is performed by mesoderm induction and embryonic body formation, with cells produced for up to 4 months.
Phase contrast images:
Phase contrast images of iPSC derived microglia- and macrophage-like cells following 7 day maturation.
Immunocytochemistry of iPSC-derived microglia-like cells stained for classic markers.
Cell surface receptor expression measured by flow cytometry. Each batch of cells generated is quality control tested by measuring expression of cell surface receptors.
Blue: Marker of interest